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Endotoxin

Ddddd Residual endotoxin contamination in advanced biotherapy products is an expensive and often difficult contaminant to control. Many commercially-available protocols are unable to remove endotoxins effectively and are based on non-affinity chromatography methods e.g. Ion exchange chromatography, phase separation using Triton X-114 or require time consuming and expensive affinity steps. These costly resins are often supplied as loose resin or packed in slow gravity columns.

Next generation Proteus kits combine the quality separation you expect from gravity flow columns with the speed and ease-of-use of spin columns to give you 4-log reductions of endotoxin without compromising protein or antibody yields. The proprietary FlowGo technology regulates sample movement through the high-quality affinity resin matrix, increasing both endotoxin removal and protein recovery. Uniquely, we offer flow rate control for endotoxin removal in a centrifuge.

The NoEndo (Micro) and NoEndo M (Mini) spin columns incorporate a SelfSeal membrane technology which retains the NoEndo resin and sample in the batch incubation chamber. The NoEndo μ are suitable for microfuges and can accommodate sample volumes up to 0.6 ml and the NoEndo M spin columns can accomodate sample volumes up to 20 ml. The membrane is specially formulated to prevent any sample from leaking into the collection tube on an orbital mixer. In a centrifuge, the membrane pores dilate and the eluate, free of endotoxin, passes into the collection tube. The contact time is maximized to ensure maximum endotoxin depletion without losses of the target protein, antibody or domain antibody.

Kod producenta Pełna nazwa Ilość
GEN-NoE24Micro Proteus NoEndoµ (Micro) (includes 2.4 ml NoEndo™ resin vial) 24 24 column kit
GEN-NoE12M Proteus NoEndoM (Mini) (includes 12 x 0.25 ml NoEndo™ resin vials) 12 12 column kit
GEN-NoE12S Proteus NoEndoS (Standard) 12 12 column kit
GEN-NoE12HC Proteus NoEndoHC (High Capacity) 12 12 column kit
GEN-NoE100Micro Proteus NoEndoµ (Micro) (includes 10.0 ml NoEndo™ resin vial) 100 100 column kit
GEN-NoE48M Proteus NoEndoM (Mini) (includes 48 x 0.25 ml NoEndo™ resin vials) 48 48 column kit
GEN-NoE48S Proteus NoEndoS (Standard) 48 48 column kit
GEN-NoE48HC Proteus NoEndoHC (High Capacity) 48 48 column kit
GEN-NoE025ML 25 ml NoEndo Resin (1 x 25 ml; 50% slurry) 25 ml
GEN-NoE100ML 100 ml NoEndo Resin (1 x 100 ml; 50% slurry) 100 ml

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